Cyandye Lot 10002說明書

　更新時間：2018-06-21　點擊量：1771

上海起發為Cyandye特約經銷商，Cyandye是一家以合理的價格向處理分子生物學的科學家提供化學服務的公司。Cyandye的專長是生物技術和生命科學的熒光染料。

Cyandye Lot 10002說明書：

貨號	品名	規格	品牌
Lot 10002	Azidobutyrate NHS Ester	50 mg	cyandye
Lot 10002	Azidobutyrate NHS Ester	100 mg	cyandye
Lot 10002	Azidobutyrate NHS Ester	250 mg	cyandye
Lot 10002	Azidobutyrate NHS Ester	50 mg	cyandye

分子式：C 8 H 10 N 4 O 4分子量
：226.19
同義詞：

1 - [（4- azidobutanoyl）氧基]吡咯烷-2,5-二酮
琥珀酰亞胺4-疊氮丁酸酯
NHS-C3疊氮
疊氮丁酸氧基琥珀酰亞胺酯>

對于標記0.2微摩爾的氨基修飾的寡核苷酸，在60μL的ACN中溶解2.3mg的疊氮丁酸NHS酯（DMSO或DMF也是可接受的）。將氨基修飾的寡核苷酸溶解在500μL的0.1M碳酸鹽/碳酸氫鹽緩沖液（pH 9）中。加入12μL的Azidobutyrate NHS酯溶液到溶解的寡核苷酸中，并在室溫下反應2小時并脫鹽。

儲存：干燥，-10至-30°C
稀釋劑：無水ACN，DMSO或DMF溶液
穩定性：2-3天

質量控制： NMR：95％TLC：純

AZIDOBUTYRATE使用

在這種情況下，在任何合成器上像往常一樣合成寡核苷酸。氨基必須在寡核苷酸的3'或5'端引入。在裂解和脫保護之后，疊氮基可以作為胺的修飾被引入。例如，碳酸鈉/碳酸氫鈉緩沖液（pH8.75）中的寡核苷酸在室溫下與琥珀酰亞胺基-4-疊氮基丁酸酯在DMSO中孵育。然后使用HPLC純化終的寡核苷酸或用乙醇或丁醇沉淀。

參考文獻：
J. Am。化學。
使用Click Chemistry
Langmuir，2010，26（11），pp 8666-8672 DOI：10.1021 / ja070273v Template-Directed Oligonucleotide Strand Ligation，Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry Langmuir，2010，26（11），pp 8666-8672 DOI： 10.1021 / la904822f
將脂質囊泡共價連接到液體支持的雙層允許觀察DNA介導的囊泡相互作用

Cyandye 產品列表：

貨號	品名	規格	品牌
Lot 10002	Azidobutyrate NHS Ester	50 mg	cyandye
Lot 10002	Azidobutyrate NHS Ester	100 mg	cyandye
Lot 10002	Azidobutyrate NHS Ester	250 mg	cyandye
Lot 10007	Cy3 carboxylic acid	5 mg	cyandye
Lot 10007	Cy3 carboxylic acid	100 mg	cyandye
Lot 10006	Cy5 carboxylic acid	5 mg	cyandye
Lot 10012	Cy5.5 carboxylic acid	5 mg	cyandye
Lot 10012	Cy5.5 carboxylic acid	25 mg	cyandye
Lot 10012	Cy5.5 carboxylic acid	50 mg	cyandye
Lot 10012	Cy5.5 carboxylic acid	100 mg	cyandye
Lot 10013	diSulfo - Cy3 alkyne	1 mg	cyandye
Lot 10013	diSulfo - Cy3 alkyne	5 mg	cyandye
Lot 10013	diSulfo - Cy3 alkyne	10 mg	cyandye
Lot 10013	diSulfo - Cy3 alkyne	25 mg	cyandye
Lot 10022	diSulfo - Cy3 azide	1 mg	cyandye
Lot 10022	diSulfo - Cy3 azide	5 mg	cyandye
Lot 10022	diSulfo - Cy3 azide	10 mg	cyandye
Lot 10022	diSulfo - Cy3 azide	25 mg	cyandye
Lot a0015	diSulfo - Cy3 carboxylic acid	5 mg	cyandye
Lot a0015	diSulfo - Cy3 carboxylic acid	25 mg	cyandye
Lot a0015	diSulfo - Cy3 carboxylic acid	50 mg	cyandye
Lot a0015	diSulfo - Cy3 carboxylic acid	100 mg	cyandye
Lot 10017	diSulfo - Cy3 NHS Ester	5 mg	cyandye
Lot 10017	diSulfo - Cy3 NHS Ester	25 mg	cyandye
Lot 10017	diSulfo - Cy3 NHS Ester	50 mg	cyandye
Lot 10017	diSulfo - Cy3 NHS Ester	100 mg	cyandye
Lot 10016	diSulfo - Cy5 alkyne	1 mg	cyandye
Lot 10016	diSulfo - Cy5 alkyne	5 mg	cyandye
Lot 10016	diSulfo - Cy5 alkyne	10 mg	cyandye
Lot 10016	diSulfo - Cy5 alkyne	25 mg	cyandye
Lot 10023	diSulfo - Cy5 azide	1 mg	cyandye
Lot 10023	diSulfo - Cy5 azide	5 mg	cyandye
Lot 10010	diSulfo - Cy5 carboxylic acid	5 mg	cyandye
Lot 10010	diSulfo - Cy5 carboxylic acid	100 mg	cyandye
Lot 10009	diSulfo - Cy5 Hydrazide	5 mg	cyandye
Lot 10009	diSulfo - Cy5 Hydrazide	25 mg	cyandye
Lot 10009	diSulfo - Cy5 Hydrazide	50 mg	cyandye
Lot 10009	diSulfo - Cy5 Hydrazide	100 mg	cyandye
Lot 10014	diSulfo - Cy5 NHS Ester	5 mg	cyandye
Lot 10014	diSulfo - Cy5 NHS Ester	25 mg	cyandye
Lot 10014	diSulfo - Cy5 NHS Ester	50 mg	cyandye
Lot 10014	diSulfo - Cy5 NHS Ester	100 mg	cyandye
Lot 10027	diSulfo - Cy7 carboxylic acid	5 mg	cyandye
Lot a0021	diSulfo - Cy7 NHS Ester	5 mg	cyandye
Lot a0021	diSulfo - Cy7 NHS Ester	25 mg	cyandye
Lot a0021	diSulfo - Cy7 NHS Ester	50 mg	cyandye
Lot a0021	diSulfo - Cy7 NHS Ester	100 mg	cyandye
Lot 10004	monoSulfo - Cy3 alkyne	1 mg	cyandye
Lot 10004	monoSulfo - Cy3 alkyne	5 mg	cyandye
Lot 10004	monoSulfo - Cy3 alkyne	10 mg	cyandye
Lot 10004	monoSulfo - Cy3 alkyne	100 mg	cyandye
Lot 10011	monoSulfo - Cy3 azide	1 mg	cyandye
Lot 10011	monoSulfo - Cy3 azide	5 mg	cyandye
Lot 10011	monoSulfo - Cy3 azide	10 mg	cyandye
Lot 10011	monoSulfo - Cy3 azide	100 mg	cyandye
Lot 10025	monoSulfo - Cy3 carboxylic acid	5 mg	cyandye
Lot 10025	monoSulfo - Cy3 carboxylic acid	25 mg	cyandye
Lot 10025	monoSulfo - Cy3 carboxylic acid	50 mg	cyandye
Lot 10025	monoSulfo - Cy3 carboxylic acid	100 mg	cyandye
Lot 10008	monoSulfo - Cy3 NHS Ester	5 mg	cyandye
Lot 10008	monoSulfo - Cy3 NHS Ester	100 mg	cyandye
Lot 10020	monoSulfo - Cy5 azide	1 mg	cyandye
Lot 10020	monoSulfo - Cy5 azide	5 mg	cyandye
Lot 10020	monoSulfo - Cy5 azide	10 mg	cyandye
Lot 10020	monoSulfo - Cy5 azide	100 mg	cyandye
Lot 10026	monoSulfo - Cy5 carboxylic acid	5 mg	cyandye
Lot 10026	monoSulfo - Cy5 carboxylic acid	25 mg	cyandye
Lot 10018	monoSulfo - Cy5 NHS Ester	5 mg	cyandye
Lot 10018	monoSulfo - Cy5 NHS Ester	25 mg	cyandye
Lot a0024	3-azidopropylamine	100 mg	cyandye
Lot d2501	2'-Deoxy-5'-acetyl-5-acetoxymethyluridine	250 mg	cyandye
Lot a0001	2'-Deoxy-5-hydroxymethyluridine	250 mg	cyandye
Lot a0001	2'-Deoxy-5-hydroxymethyluridine	500 mg	cyandye
Lot a0029	4-hydrazinobenzene-1,3-disulfonic acid	500 mg	cyandye
Lot a0028	5-(hydroxymethyl)-1-[(6aR,8R,9aS)-2,2,4,4-tetraisopropyltetrahydro-6H-furo[3,2-f][1,3,5,2,4]trioxadisilocin-8-yl]pyrimidine-2,4(1H,3H)-dione	50 mg	cyandye
Lot a0019	2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-ol	500 mg	cyandye
Lot a0003	2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose	50 mg	cyandye
Lot a0003	2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose	100 mg	cyandye
Lot a0003	2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose	250 mg	cyandye

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